It refers to sequencing the entire genome of an
organism, either de novo or reference based.
Resequencing is done for Genotyping and
Variation analysis, with the help of known reference sequences.
De novo sequencing refers to sequencing a novel
genome where there is no reference sequence available for alignment. Sequence reads are assembled as
contigs, and the coverage quality of de novo sequence data depends on the size and continuity of the
contigs (ie, N50 size and the number of gaps in the data).
Hybrid sequencing utilizes various approaches
like short read sequencing, Long read sequencing, Genome Mapping, Chromosome conformation
technologies to achieve accurate Genome assembly.
Mitochondrial DNA sequencing helps in Population
Genetics and disease studies, as it is passed from mother to child and is responsible for a variety