Small RNA sequencing is another next-generation sequencing-based technology that sequences small RNA species, used to examine and characterize variations in miRNAs and discover novel microRNAs (miRNAs). It is a powerful method for comprehensive understanding of post-transcriptional regulation contributing to phenotype.

• Profiling of small RNAs and microRNAs.
• Understanding the role of post-transcriptional regulation in phenotype. 
• More un-mapped short RNAs and isoforms, as well as novel biomarkers, are being sought.

Bioinformatics analysis of small RNA include controlling the quality of raw data and applying a length filter, Mapping based on references, Classification and measurement of small RNA , Annotation and prediction of target genes , Prediction of a novel small RNA , Analysis of differentially expressed small RNA’s target genes (GO enrichment and KEGG enrichment).