Small RNA sequencing is another next-generation sequencing-based technology that sequences small RNA species, used to examine and characterize variations in miRNAs and discover novel microRNAs (miRNAs). It is a powerful method for comprehensive understanding of post-transcriptional regulation contributing to phenotype.

  • Profiling of small RNAs and microRNAs.
  • Understanding the role of post-transcriptional regulation in phenotype. 
  • More un-mapped short RNAs and isoforms, as well as novel biomarkers, are being sought.

Bioinformatics analysis of small RNA include controlling the quality of raw data and applying a length filter, Mapping based on references, Classification and measurement of small RNA , Annotation and prediction of target genes , Prediction of a novel small RNA , Analysis of differentially expressed small RNA’s target genes (GO enrichment and KEGG enrichment).

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