mRNA sequencing (mRNA-Seq) has become one of the best methods for analyzing the transcriptomes of disease states, of biological processes along with a wide range of study designs. Being a highly sensitive and accurate means of quantifying gene expression, mRNA-Seq identifies both known and novel transcript isoforms, gene fusions, and allele-specific expression. mRNA-Seq provides a complete view of the coding transcriptome, not being restricted by the used filters till now.
mRNA sequencing provides an accurate, high-resolution view of the transcriptome.
Advantages of mRNA-Seq over gene expression arrays in analyzing the transcriptome are:
- Detects sensitive and accurate measurement of fold changes in gene expression over dynamic range
- mRNA-Seq can capture both known and novel features
- It is applicable over wide range of species